Ferritin Heavy Chain Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS90501-50, BS90501-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P02794(Human) P09528(Mouse) P19132(Rat) Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IHC ICC All Applications: WB:1:500-1:1,000 IHC:1:50-1:200 ICC:1:50-1:200 Background:  Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.  Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or  -80°C. Avoid repeated freeze / thaw cycles. Specificity: Ferritin Heavy Chain polyclonal antibody detects endogenous levels of Ferritin Heavy Chain protein. Bioworld Molecular Weight: 21 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: ICC staining Ferritin Heavy Chain in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Ferritin Heavy Chain in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton Alternative Name: Ferritin H subunit, FTH1, FTH, FTHL6, Apoferritin, Cell proliferation inducing gene 15 protein, Cell proliferation-inducing gene 15 protein, F HC, Ferritin H subunit, Ferritin heavy chain, Ferritin heavy polypeptide 1, FHC, FRIH, FRIH, FTH 1, FTH, FTH1, FTH1 protein, FTHL 6, FTHL6, Iron overload autosomal dominant, MGC104426, N-terminally processed, OK/SW-cl.84, PIG 15, PIG15, Placenta immunoregulatory factor, PLIF, Proliferation inducing gene 15 protein, Proliferation inducing protein 15, Immunogen: Recombinant protein Conjugate: Unconjugated Modification: Unmodified