Ferritin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90500-50, BS90500-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P02792(Human) P02794(Human) P09528(Mouse) P29391(Mouse) P02793(Rat) P19132(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, zebrafish
Applications: WB, ICC/IF
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
Background: Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Ferritin polyclonal antibody detects endogenous levels of Ferritin protein.
Bioworld Molecular Weight: 21 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Ferritin on zebrafish lysates using anti-Ferritin antibody at 1/1,000 dilution., ICC staining Ferritin in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Ferritin H subunit, FTH1, Ferritin L subunit, FTL, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light polypeptide, Ferritin, heavy polypeptide, FRIH, FTH, FTH1, FTL,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified