Product Description

FFAR3 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-90435-60, E-AB-90435-120, E-AB-90435-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: FFAR3

Target Synonym: FFAR3; FFA3R; GPR41; GPR42

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Mouse, Rat

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O14843

Background: G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs, and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o-alpha family of G proteins. Its activation results in the formation of inositol 1, 4, 5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. Activated by SCFAs and by beta-hydroxybutyrate, a ketone body produced by the liver upon starvation, it inhibits N-type calcium channels and modulates the activity of sympathetic neurons through a signaling cascade involving the beta and gamma subunits of its coupled G protein, phospholipase C and MAP kinases. Thereby, it may regulate energy expenditure through the control of the sympathetic nervous system that controls for instance heart rate.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human FFAR3

Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 38kDa

Observed MW: 39KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only