FHL2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90510-50, BS90510-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q14192(Human) O70433(Mouse) O35115(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP,
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
IP:1:10-1:50
Background: The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2) and FHL-4. The signature "half-domain", a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. Skeletal and cardiac muscle express FHL-1 in high levels as compared to the low level of expression in smooth muscle of the colon, small intestine and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes. FHL-2 is also located in cardiac and skeletal muscle, as well as in placenta and ovary tissues. FHL-3 is found in skeletal muscle, but absent in cardiac muscle. FHL-4 is expressed exclusively by the seminiferous epithelium of the testis, which suggests that FHL-4 is involved in spermatogenesis. The genetic loci for FHLs vary considerably despite similiar amino acid sequences among the FHL group.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: FHL2 polyclonal antibody detects endogenous levels of FHL2 protein.
Bioworld Molecular Weight: 32 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of FHL2 on different tissue lysates using anti-FHL2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat heart
Lane 2: Mouse heart, Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.
Alternative Name: FHL-2, SLIM-3, FHL2, DRAL, SLIM3, AAG 11, AAG11, Aging associated gene 11, Down regulated in rhabdomyosarcoma LIM protein, Downregulated in rhabdomyosarcoma LIM protein, DRAL, FHL 2, FHL-2, Fhl2, FHL2 protein, FHL2, Four and a half LIM domain protein 2, Four and a half LIM domains 2, Four and a half LIM domains protein 2, KIAA0990, LIM domain protein DRAL, Skeletal muscle LIM protein 3, Skeletal muscle LIM-protein 3, SLIM 3, SLIM-3, SLIM3,
Immunogen: Recombinant protein within C-terminal human FHL2.
Conjugate: Unconjugated
Modification: Unmodified