Product Description

Fibrillarin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90511-50, BS90511-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P22087(Human) P35550(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, FC

All Applications: WB:1:500-1:1,000
FC:1:50-1:100

Background: Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Fibrillarin polyclonal antibody detects endogenous levels of Fibrillarin protein.

Bioworld Molecular Weight: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Fibrillarin on different cell lysates using anti-Fibrillarin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: 293T
Lane 4: NIH-3T3, Flow cytometric analysis of HL-60 cells with Fibrillarin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: FBL, FIB1, FLRN, 34 kD nucleolar scleroderma antigen, 34 kDa nucleolar scleroderma antigen, Fbl, FBRL, FIB, FIB1, FLRN, Histone-glutamine methyltransferase, Nop1p, RNA U3 small nucleolar interacting protein 1, RNU3IP1, rRNA 2' O methyltransferase fibrillarin, rRNA 2'-O-methyltransferase fibrillarin,

Immunogen: Synthetic peptide of C-terminal human Fibrillarin.

Conjugate: Unconjugated

Modification: Unmodified