Product Description

FIH-1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67227-50, MB67227-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: FIH (Factor inhibiting HIF-1, HIF asparagine hydroxylase) is a dioxygen-dependent asparaginyl hydroxylase that modifies target protein function by hydroxylating target protein asparagine residues. Hypoxia-inducible factor (HIF), a transcriptional activator involved in control of cell cycle in response to hypoxic conditions, is an important target for FIH regμlation. FIH functions as an oxygen sensor that regμlates HIF function by hydroxylating at Asn803 in the carboxy-terminal transactivation domain (CAD) of HIF. During normoxia, FIH uses cellμlar oxygen to hydroxylate HIF-1 and prevent interaction of HIF-1 with transcriptional coactivators, including the CBP/p300-interacting transactivator. Under hypoxic conditions, FIH remains inactive and does not inhibit HIF, allowing the activator to regμlate transcription of genes in response to low oxygen conditions. FIH activity is regμlated in through interaction with proteins, including Siah-1, which targets FIH for proteasomal degradation. The Cut-like homeodomain protein CDP can bind the FIH promoter region to regμlate FIH expression at the transcriptional level. Phosphorylation of HIF at Thr796 also can prevent FIH hydroxylation on Asn803. Potential FIH substrates also include proteins with ankyrin repeat domains, such as Iκ-B, Notch, and ASB4.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: FIH1, Hypoxia-inducible factor 1-alpha inhibitor, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FIH-1. The exact sequence is proprietary.

Dilution: WB (1/1000 - 1/2000), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of FIH-1 protein.

Molecular Weight: ~ 42 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NWT6

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only