Filamin 1 (phospho-S2152) Polyclonal Antibody-BS4075

Filamin 1 (phospho-S2152) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4075-50, BS4075-100

Swiss-Prot: P21333

Host: Rabbit

Filamin 1 (phospho-S2152) Polyclonal Antibody-BS4075

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Filamin 1 (S2152) polyclonal antibody detects endogenous levels of Filamin 1 protein when phosphorylated at Ser2152.

Bioworld Molecular Weight: ~ 280 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Filamin 1 (phospho-S2152) polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (20ug)
Lane 3: The Lung tissue lysate of Rat (20ug)
Lane 4: AML-12 whole cell lysate (40ug)
Lane 5: CT26 whole cell lysate (40ug)
Lane 6: SKOVCAR3 whole cell lysate (40ug)
Lane 7: Panc1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Filamin 1 (S2152) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: FilaminA; Filamin A; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Filamin1; Non-muscle filamin; FLNA; FLN; FLN1

Immunogen: Synthetic phosphopeptide derived from human Filamin 1 around the phosphorylation site of Serine 2152.

Modification: Phosphorylation

Conjugate: Unconjugated