Filamin 1 Rabbit Monoclonal Antibody-BS9826M

Filamin 1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9826M-50, BS9826M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21333

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:5000
IHC/ICC/IF: 1:50-1:200
FC: 1:10-1:100

Background: Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Filamin 1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 280 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Filamin 1 Rabbit mAb at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: NIH/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin 1 antibody. Counter stained with hematoxylin., ICC staining Filamin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: FilaminA; Filamin A; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Filamin1; Non-muscle filamin; FLNA; FLN; FLN1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified