Product Description

FIR (P364) Polyclonal Antibody

Catalogue Numbers: BS1895-50, BS1895-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O94887

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: FARP2 (FERM, RhoGEF and pleckstrin domain-containing protein 2), also known as PLEKHC3 or FERM domain including RhoGEF (FIR), is a 1,545 amino acid protein that contains one FERM domain, one DH domain and two PH domains. It exists as two alternatively spliced isoforms that are abundantly expressed in brain, lung, and testis as well as in embryonic hippocampal and cortical neurons. FARP2 functions as a Rho-guanine nucleotide exchange factor that activates RAC1 and is thought to regulate neurite remodeling of embryonic neurons. Sema3A binding to neuropilin-1 induces the dissociation of FARP2 from plexin-A1, thereby activating FARP2's Rac GEF activity which is critical for repulsion of outgrowing axons and suppression of neuronal adhesion. Downregulation of the FARP2 gene has been implicated in autism.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FIR (P364) polyclonal antibody detects endogenous levels of FIR protein.

Extra Notes: Western blot (WB) analysis of FIR (P364) pAb at 1:1000 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:PC12 whole cell lysate(40ug)
Lane4:AML-12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: FERM, RhoGEF and pleckstrin domain-containing protein 2; FERM domain including RhoGEF; FIR; Pleckstrin homology domain-containing family C member 3; PH domain-containing family C member 3; FARP2; KIAA0793; PLEKHC3

Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human FIR.

Conjugate: Unconjugated

Modification: Unmodified