Firefly Luciferase Rabbit Monoclonal Antibody-BS40130

Firefly Luciferase Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS40130-50, BS40130-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P08659

Host: Rabbit

Reactivity: Firefly

Applications: WB, ICC/IF

All Applications: WB 1:500~1:2000 ICC/IF 1:50~1:200

Background: Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Purification and Purity: Affinity-chromatography

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Firefly Luciferase Antibody detects endogenous levels of total Firefly Luciferase

Bioworld Molecular Weight: 55kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Firefly Luciferase Antibody - N-terminal expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate., Immunofluorescent analysis of 293 cells transfected with Firefly Luciferase, using Firefly Luciferase Antibody.

Alternative Name: Luciferase; Firefly; Luciferin 4 monooxygenase;

Immunogen: A synthesized peptide derived from Firefly Luciferase

Conjugate: Unconjugated

Modification: Unmodified