Flag-Tag (HRP Conjugated) Mouse Monoclonal Antibody - EM1024

Flag-Tag (HRP Conjugated) Mouse Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: EM1024-50, EM1024-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Host: Mouse

Applications: WB

Alternate Names: DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag

Clonality: Monoclonal

Immunogen: Synthetic Peptide

Isotype: IgG1

Dilution: WB 1:5, 000-10, 000

Concentration: 1mg/mL

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Research Use Only