Flag-Tag Monoclonal Antibody - E-AB-20006

Flag-Tag Monoclonal Antibody Sizes: 60μL, 120μL, 200μL Catalogue Numbers: E-AB-20006-60, E-AB-20006-120, E-AB-20006-200 Citations, Manuals and MSDS Available upon request. Abbreviation: Flag-Tag Target Synonym: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag Research Areas: Tags and Cell Markers Conjugation: Unconjugated Host: Mouse Application: WB, IF, IP Isotype: IgG Clonality: Monoclonal Clone No.: Clone:4A2 Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.  Concentration: 1 mg/mL Immunogen: Synthetic Peptide Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Purification Method: Protein A purification Dilution: WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300 Storage: -20°C/One year. Avoid freeze / thaw cycles. Shipping: Ice bag Research Use Only