Product Description

Flag-Tag Monoclonal Antibody

Sizes: 50μL, 100μL, 200μL

Catalogue Numbers: E-AB-48025-50, E-AB-48025-100, E-AB-48025-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Flag-Tag

Target Synonym: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Conjugation: Unconjugated

Host: Mouse

Species Reactivity: All

Application: WB, IF, IP, FCM

Isotype: IgG2a

Clonality: Monoclonal

Clone No.: 3H9

Background: FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.

Concentration: 1mg/mL

Immunogen: Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.4

Purification Method: Protein A/G Purified

Dilution: WB: 1:5000-10000; IF: 1:500-2000, IP: 3ug/sample, FCM:0.2 ug

Calculated MW: 45kDa

Observed MW: 45kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only