Product Description

FLAG-tag Polyclonal Antibody

Catalogue Numbers: BS67268-50, BS67268-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Host: Rabbit

Applications: WB, IP

All Applications: WB (1/1000 - 1/2000), IP (1/10 - 1/100)

Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes the FLAG-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Extra Notes: Western blot analysis of Anti-FLAG-tag Antibody against HEK293T cells transfected with vector overexpressing FLAG tag (A) and untransfected (B).

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Immunogen: KLH-conjugated synthetic peptide DYKDDDDK.

Conjugate: Unconjugated

Modification: Unmodified