FLAG-tag Polyclonal Antibody - BT-AP15612

FLAG-tag Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP15612-20, BT-AP15612-50, BT-AP15612-100

Citations, Manuals and MSDS Available upon request.

Background: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Research Areas: Tags and cell markers

Target Protein: Flag

Applications: WB, ELISA

Reactivity: Species independent

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: Flag-Tag DDDDK TAG, DDDDK-TAG

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Synonyms: Flag-Tag DDDDK TAG, DDDDK-TAG; DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Research Use Only