Product Description

Flag-Tag Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-20045-60, E-AB-20045-120, E-AB-20045-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Flag-Tag

Target Synonym: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Research Areas: Tags and Cell Markers

Conjugation: Unconjugated

Host: Rabbit

Application: WB

Isotype: IgG

Clonality: Polyclonal

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.

Concentration: 1 mg/mL

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Affinity purification

Dilution: WB 1:5000-20000

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only