Product Description

FLAG-tag Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA20821-50, RA20821-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Reactivity: Species independent

Host: Rabbit

Applications: WB, ELISA

Alternate Names: DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag

Clonality: Polyclonal

Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.

Storage: -20°C/1 year

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Research Use Only