Product Description

FLOT2 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS8274-50, BS8274-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents. Lipid rafts are important for numerous cellμlar processes, including signal transduction, membrane trafficking and molecμlar sorting. Flotillins are lipid raft components in neurons and caveloae-associated proteins in A498 kidney cells. Flotillin-2, also designated epidermal surface antigen, is conserved in all mammalian species. Flotillin-1 and -2 have complementary tissue distributions and their expression levels are independently regμlated. At the cellμlar level, Flotillin-2 is ubiquitously expressed, whereas Flotillin-1 is expressed in A498 kidney cells, muscle cell lines and fibroblasts. Stable transfection of a Flotillin-2 fusion protein in COS cells induces filopodia formation and changes epithelial cells to a neuronal appearance. Flotillins form a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane. Association of ER-X with Flotillin localizes ER-X within plasma membrane caveloae and mediates rapid oestrogen activation of the MAP kinase cascade. The expression of the flotillins is also correlated to the progression of Alzhemier pathology.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: Flotillin-2, Epidermal surface antigen, ESA, Membrane component chromosome 17 surface marker 1, FLOT2, ESA1, M17S1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length Human FLOT2.

Dilution: WB: 1:500~1:2000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: FLOT2 polyclonal antibody detects endogenous levels of FLOT2 protein.

Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q14254

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only