Flotillin-2 (R144) Polyclonal Antibody-BS2878

Flotillin-2 (R144) Polyclonal Antibody

Catalogue Numbers: BS2878-50, BS2878-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14254

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization. Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome. Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Flotillin-2 (R144) polyclonal antibody detects endogenous levels of Flotillin-2 protein.

Extra Notes: Western blot (WB) analysis of FLOT2 pAb at 1:500 dilution
Lane1:A375 whole cell lysate(40ug)
Lane2:SCC-7 whole cell lysate(40ug)
Lane3:The Brain tissue lysate of Mouse(40ug)
Lane4:C6 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Flotillin-2; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1; ESA1; M17S1; FLOT2

Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Conjugate: Unconjugated

Modification: Unmodified