FMN1 Polyclonal Antibody-BS65749

FMN1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65749-50, BS65749-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q68DA7

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Cow, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. In mice, the limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of proteins known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing Actin filament assembly. Formins co-localize with the actin cytoskeleton and can translocate into the cell cytosol and into the nucleus in an HGF-dependent manner. Vertebrate nuclear formins can control polarizing activity in limb buds through establishment of a Sonic hedgehog/FGF-4 feedback loop. Deficiency mutations at the mammalian ld locus lead to profound developmental defects in limb and kidney formation. The human Formin 1 and 2 genes map to chromosome 15q13.3 and 1q43, respectively.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FMN1 Polyclonal Antibody detects endogenous levels of FMN1 protein.

Bioworld Molecular Weight: 157kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated (bs-13185R) at 1:200 overnight at 4°C

Alternative Name: FMN; Formin 1; Formin1; Formin-1; LD; Limb deformity protein homolog; FMN1_HUMAN.聽

Immunogen: KLH conjugated synthetic peptide derived from human FMN1/Formin 1:651-750/1419

Conjugate: Unconjugated

Modification: Unmodified