FMRP Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90520-50, BS90520-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q06787(Human) P35922(Mouse) Q80WE1(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:5,000-1:10,000
ICC:1:100-1:500
IHC:1:50-1:200
Background: Fragile X syndrome is the most frequent form of inherited mental retardation and is the result of transcriptional silencing of the FMR1 gene on the X chromosome. The FMR1 gene contains a distinct CpG dinucleotide repeat located in the 5' untranslated region of the gene. In fragile X syndrome this tandem repeat is substantially amplified and subjected to extensive methylation and enhanced transcriptional silencing. The FMR1 protein (or FMRP) is an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. It contains several features that are characteristics of RNA-binding proteins, including two hnRNPK homology (KH) domains and an RGG amino acid motif (RGG box). FMR1 localizes to both the nucleus and the cytoplasm and can also interact with two fragile X syndrome related factors, FXR1 and FXR2, which form heterodimers through their N-terminal coiled-coil domains. Since FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in the nucleocytoplasmic transport of mRNAs.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: FMRP polyclonal antibody detects endogenous levels of FMRP protein.
Bioworld Molecular Weight: 71 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of FMRP on different cells lysates using anti-FMRP antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562, ICC staining FMRP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: FMRP, Protein FMR-1, FMR1, FMR 1, Fmr1, Fmr1 gene, FMR1, FMRP, Fragile X mental retardation 1, Fragile X mental retardation 1 protein, Fragile X mental retardation protein 1, Fragile X mental retardation protein, fragile X mental retardation syndrome-related protein 1, fragile X mental retardation, autosomal homolog 1, FRAXA, fxr1, MGC87458, POF, POF1, Protein FMR-1, Protein FMR1, wu:fb16f11, wu:fd18c10, zgc:66226,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified