FNDC3A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90521-50, BS90521-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: F1R5M9 Zebrafish
Host: Rabbit
Reactivity: Zebrafish
Applications: IF, IHC
All Applications: IF:1:50-1:200
IHC:1:50-1:200
Background: Fibronectin type III domain containing 7, or, FNDC7, is a human gene. Other names include: Fibronectin type III domain-containing protein 7. The encoded protein has an amino acid length of 733 and a mass of 78.2 kDa. Other isoforms may exist. This gene shares homology with other species, including: Mouse, Rat, Zebrafish, Frog, Chicken.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: FNDC3A polyclonal antibody detects endogenous levels of FNDC3A protein.
Bioworld Molecular Weight: 67 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-66 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor and trade; 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature., Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-66 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Alternative Name: fndc7a, Fibronectin type III domain containing protein 3a, FNDC3, HUGO, KIAA0970 antibody
Immunogen: Recombinant protein within Zebrafish FNDC3A aa 100-310.
Conjugate: Unconjugated
Modification: Unmodified