Product Description

Focal Adhesion Kinase (Phospho-Y397) Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66304-50, MB66304-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis . This protein kinase is activated by insμlin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad , forkhead transcription factors, c-Raf , and caspase-9. PTEN phosphatase is a major negative regμlator of the PI3K/Akt signaling pathway . LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regμlation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insμlin stimμlation of glucose transport . In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regμlation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regμlating the cyclin-dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1 . Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

Category: Primary Antibody

Reactivity: Rat

Host: Rabbit

Applications: WB

Alternate Names: FAK, FAK1, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regμlatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Focal Adhesion Kinase (pY397) protein.

Molecular Weight: ~ 120 kDa

Pathway: AMPK Signaling Pathway, Adherens Junction signaling, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer\'s Disease, Tumor Angiogenesis, Regμlation of Apoptosis, Tumor Angiogenesis, ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, Jak Stat: IL-6 Receptor Signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Regμlation of Microtubμle Dynamics, Mitochondrial Control of Apoptosis, NF-kB Signaling, PI3K AKT signaling, Protein Kinase C Signaling, T cell receptor signaling, TGFβ Smad signaling, Translational Contral elF4 and p70 S6 Kinase, Warburg Effect, MTOR signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O35346

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only