Fodrin α Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90523-50, BS90523-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13813(Human) P16546(Mouse) P16086(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
Background: Alpha Fodrin also named Spectrin. Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous α and β chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin α I and spectrin β I are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrin β II (also designated fodrin β) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin α II, a neuronal cytoskeleton protein.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Fodrin α polyclonal antibody detects endogenous levels of Fodrin α protein.
Bioworld Molecular Weight: 285/150 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Alpha Fodrin on different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: NIH-3T3
Lane 4: Rat brain
Lane 5: Mouse brain, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
Alternative Name: NEAS, SPTA2, (ALPHA)II-SPECTRIN, Alpha II Spectrin, Alpha-II spectrin antibodybrain, EIEE5, FLJ17738, FLJ44613, Fodrin alpha chain, Fodrin, alpha, NEAS, Non erythrocytic spectrin alpha, non-erythroid alpha chain, SPECA, Spectrin alpha chain, Spectrin alpha chain brain, Spectrin alpha non erythrocytic 1, Spectrin, Spectrin non erythroid alpha chain, Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Spectrin, nonerythroid, alpha subunit, Spna2, SPTA 2, SPTA2, SPTA2, SPTAN 1, SPTAN1,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified