Product Description

Fos B (S23) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1645-50, BS1645-100

Sizes: 50µl, 100µl

Swiss-Prot: P53539

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

Background: Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: FosB (S23) polyclonal antibody detects endogenous levels of FosB protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Fos B (S23) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of FosB (S23) pAb in paraffin-embedded human prostate carcinoma tissue.

Bioworld Molecular Weight: ~ 35 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Protein fosB; G0/G1 switch regulatory protein 3; FOSB; G0S3

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Fos B.

Conjugate: Unconjugated

Modification: Unmodified