FOXA1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90525-50, BS90525-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P55317(Human) P35582(Mouse) P23512(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: FOXA1 polyclonal antibody detects endogenous levels of FOXA1 protein.
Bioworld Molecular Weight: 49 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of FOXA1 on different lysates using anti-FOXA1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: Mouse lung, ICC staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HNF-3A, HNF-3-alpha, TCF-3A, forkhead box A1, Forkhead box protein A1, FOX A1, FOXA1, FOXA1, hepatocyte nuclear factor 3 alpha, Hepatocyte nuclear factor 3-alpha, HNF 3A, HNF-3-alpha, HNF-3A, HNF3A, MGC33105, TCF 3A, TCF-3A, TCF3A, Transcription factor 3A,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified