Product Description

FoxO1/3/4 (phospho-T24/32) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4266-50, BS4266-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors.FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regμlation of the insμlin signaling pathway. It binds to insμlin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regμlates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modμlated genes. It is also involved in negative regμlation of the cell cycle.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Forkhead box protein O4, Fork head domain transcription factor AFX1, AFX, AFX1, MLLT7, FOXO4, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FKHR, FOXO1A, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, FOXO3, FKHRL1, FOXO3A

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Threonine 24.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-FoxO1/3/4 (T24/32) polyclonal antibody detects endogenous levels of FoxO1 protein only when phosphorylated at Thr24. This antibody also detects endogenous levels of FoxO3 and FoxO4 protein only when phosphorylated at Thr32.

Molecular Weight: ~ 70 to 82 kDa

Pathway: Insμlin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q12778/O43524/P98177

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only