Product Description

FOXO1/FOXO3A/FOXO4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80182-50, BS80182-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12778/O43524/P98177

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: The Forkhead family of transcription factors mediates many aspects of physiology, including stress response, metabolism, commitment to apoptosis, and development. The Forkhead box subfamily O (FoxO) proteins have garnered particular interest due to their involvement in the modulation of cancer, metabolism and cardiovascular biology.In mammals, four subfamily members have been identified: FOXO1 (previously also known as FKHR), FOXO3 (also known as FKHRL1), FOXO4 (also known as AFX),and FOXO6.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 80kDa

Immunogen: A synthetic peptide of human FOXO1/FOXO3A/FOXO4(NP_002006.2).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using FOXO1/FOXO3A/FOXO4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded human placenta using FOXO1/FOXO3A/FOXO4 Rabbit pAb at dilution of 1:50.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse liver using FOXO1/FOXO3A/FOXO4 Rabbit pAb at dilution of 1:50.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat testis using FOXO1/FOXO3A/FOXO4 Rabbit pAb at dilution of 1:50.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.