Product Description

FOXO1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80424-50, BS80424-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12778

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 74KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using FOXO1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of HeLa cells using FOXO1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using FOXO1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: FKH1; FKHR; FOXO1A; FOXO1

Immunogen: Recombinant fusion protein of human FOXO1(NP_002006.2).

Conjugate: Unconjugated

Modification: Unmodified