FoxO1A (Acetyl Lys245) rabbit pAb - ES20066

FoxO1A (Acetyl Lys245) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES20066-50, ES20066-100

Citations, Manuals and MSDS Available upon request.

Background: disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2; 13) (q35; q14) with PAX3; translocation t (1; 13) (p36; q14) with PAX7. The resulting protein is a transcriptional activator., function:Transcription factor., PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Contains 1 fork-head DNA-binding domain., subcellular location:Shuttles between cytoplasm and nucleus., subunit:Interacts with LRPPRC., tissue specificity:Ubiquitous.,

Alternate Name: Forkhead box protein O1 (Forkhead box protein O1A; Forkhead in rhabdomyosarcoma)

Source: Rabbit

Applications: WB; ELISA

Dilution: WB 1:1000-2000 ELISA 1:5000-20000

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized peptide derived from human FoxO1A (Acetyl Lys245)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 72kD

Human Gene ID: 2308

Human SWISS Prot NO: Q12778

Subcellular Location: Cytoplasm. Nucleus. Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation

Research Use Only