FOXO1A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90529-50, BS90529-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q12778(Human) Q9R1E0(Mouse) G3V7R4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, zebrafish
Applications: WB, ICC IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: FOXO1A polyclonal antibody detects endogenous levels of FOXO1A protein.
Bioworld Molecular Weight: 80 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of FOXO1A on different lysates using anti-FOXO1A antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3, ICC staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: FOXO1, FKH 1, FKH1, FKHR, Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma), Forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, FoxO transcription factor, foxo1, FOXO1, FOXO1A, OTTHUMP00000018301,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified