Product Description

FOXO3A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90531-50, BS90531-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43524(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200

Background: FKHRL1 (forkhead in rhabdomyosarcoma-like 1), also known as FOXO3 (forkheadbox O3) or FOXO3A, is a 673 amino acid transcriptional activator thatbelongs to the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at Threonine 32 and Serine 253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephosphorylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: FOXO3A polyclonal antibody detects endogenous levels of FOXO3A protein.

Bioworld Molecular Weight: 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of FOXO3A on different lysates using anti- FOXO3A antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: PC-12
Lane 3: MCF-7, ICC staining FOXO3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: FOXO3, AF6q21, AF6q21 protein, DKFZp781A0677, FKHR2, FKHRL 1, FKHRL1, FKHRL1P2, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, Forkhead box O3, Forkhead box O3A, Forkhead box protein O3, Forkhead box protein O3A, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead homolog (rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead in rhabdomyosarcoma-like 1, FOX O3A, FOXO2, foxo3, FOXO3, FOXO3A, MGC12739, MGC31925,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified