Product Description

FoxO4 (K447) Polyclonal Antibody

Catalogue Numbers: BS1842-50, BS1842-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P98177

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FoxO4 (K447) polyclonal antibody detects endogenous levels of FoxO4 protein.

Extra Notes: Western blot (WB) analysis of FoxO4 (K447) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:Hela whole cell lysate(40ug)
Lane3:K562 whole cell lysate(40ug)
Lane4:PC12 whole cell lysate(40ug)
Lane5:3T3-L1 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Forkhead box protein O4; Fork head domain transcription factor AFX1; AFX; AFX1; MLLT7; FOXO4

Immunogen: Synthetic peptide, corresponding to amino acids 418-465 of Human FoxO4.

Conjugate: Unconjugated

Modification: Unmodified