Product Description

FPR1 (I203) Polyclonal Antibody

Catalogue Numbers: BS5720-50, BS5720-100

Sizes: 50µl, 100µl

Alternative Name: fMet-Leu-Phe receptor; fMLP receptor; N-formyl peptide receptor; Short name=FPR; N-formylpeptide chemoattractant receptor

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21462

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The N-formyl peptide receptor (FPR) is a chemotactic G protein-coupled receptor (GPCR) that is found on the surface of phagocytic leukocytes, such as neutrophils and monocytes. The human FPR family comprises three members, FPR, FPRL1 (also designated lipoxin A4 receptor) and FPRL2, and each family member contains specific residues, which are responsible for determining its ligand specificity. FPR, a seven transmembrane-domain receptor, primarily binds the chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP), which activates several biological processes, including chemotaxis, transcriptional activation, and actin reorganization. FPR also mediates the inhibition of neutrophil migration through binding to specific peptide fragments of annexin I, which causes calcium transients and affects inflammatory responses.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FPR1 (I203) polyclonal antibody detects endogenous levels of FPR1 protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FPR1 (I203) polyclonal antibody at 1:500 dilution
Lane 1: A549 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat brain tissue lysate

Immunogen: Synthetic peptide, corresponding to the 203-248 of Human FPR1.

Conjugate: Unconjugated

Modification: Unmodified