Product Description

Frizzled-4 Polyclonal Antibody

Catalogue Numbers: BS60963-50, BS60963-100

Sizes: 50µl, 100µl

Alternative Name: Frizzled-4; Fz-4; hFz4; FzE4; CD344; FZD4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9ULV1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Frizzled-4 is a 537 amino acid protein encoded by the human gene FZD4. Frizzled-4 acts as a receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-4 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Frizzled-4 also plays a critical role in retinal angiogenesis. Frizzled-4 is virtually ubiquitously expressed with greatest amounts found in adult heart, skeletal muscle, ovary, and fetal kidney.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Frizzled-4 polyclonal antibody detects endogenous levels of Frizzled-4 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Frizzled-4 pAb at 1:500 dilution
Lane 1: A549 whole cell lysate(40ug)
Lane 2: Hela whole cell lysate(40ug)
Lane 3: CT26 whole cell lysate(40ug)
Lane 4: PC12 whole cell lysate(40ug)

Immunogen: A synthetic peptide corresponding to residues in Human Frizzled-4.

Conjugate: Unconjugated

Modification: Unmodified