Frizzled-7 (Y87) Polyclonal Antibody-BS2774

Frizzled-7 (Y87) Polyclonal Antibody

Catalogue Numbers: BS2774-50, BS2774-100

Sizes: 50µl, 100µl

Alternative Name: Frizzled7; Frizzled 7; Fz-7; hFz7; FzE3; FZD7

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75084

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins: FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt. frizzled-7 has a Lys-Thr-X-X-X-Trp motif which is involved in the activation of the Wnt/beta-catenin signaling pathway. frizzled-7 is an integral membrane protein showing a high expression in adult skeletal muscle, fetal kidney, fetal lung, adult heart, brain and placenta. It is specifically expressed in squamous cell esophageal carcinomas.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Frizzled-7 (Y87) polyclonal antibody detects endogenous levels of Frizzled-7 protein.

Bioworld Molecular Weight: ~ 64, 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Frizzled-7 (Y87) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate(20ug)
Lane 2: HEK293T whole cell lysate(40ug)
Lane 3: 3T3-L1 whole cell lysate(40ug)
Lane 4: PC12 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 61-110 of Human Frizzled-7.

Conjugate: Unconjugated

Modification: Unmodified