Frizzled-8 Polyclonal Antibody-BS2700

Frizzled-8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2700-50, BS2700-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H461

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB: 1:1000~1:2000

Background: Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Frizzled-8 polyclonal antibody detects endogenous levels of Frizzled-8 protein.

Bioworld Molecular Weight: ~ 73 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Frizzled-8 (FZD8) at 1:1000 dilution
Lane 1: HEK293T whole cell lysate (30ug)
Lane 2: A549 whole cell lysate (30ug)
Lane 3: The Stomach tissue lysate of Mouse (30ug)
Lane 4: The Spleen tissue lysate of Rat (30ug)
Lane 5: BV2 whole cell lysate (30ug)
Lane 6: PC12 whole cell lysate (30ug)

Alternative Name: Fz-8; hFz8; FZD8; FZ-8; hFZ8; Frizzled 8; Frizzled8

Immunogen: Synthetic peptide, corresponding to Human Frizzled-8.

Conjugate: Unconjugated

Modification: Unmodified