Product Description

Frizzled-9 Polyclonal Antibody

Catalogue Numbers: BS5723-50, BS5723-100

Sizes: 50µl, 100µl

Alternative Name: Frizzled9; Frizzled 9; Frizzled3; Frizzled 3; Frizzled-3; Fz-9; hFz9; FzE6; CD349; FZD9; FZD3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00144

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. The frizzled-9 gene is located within the Williams Syndrome common deleted region at chromosomal band 7q11.23. Heterozygous deletion of the frizzled-9 gene may contribute to the Williams Syndrome phenotype. In mouse, frizzled-9 overexpression can induce the hyperphosphorylation and relocalization of Dvl-1 from the cytoplasm to the cell membrane and cytosolic β-catenin accumulation. In rat, frizzled-9 is important in Wnt/β-catenin signaling in 293T cells. Frizzled-9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Frizzled-9 polyclonal antibody detects endogenous levels of Frizzled-9 protein.

Bioworld Molecular Weight: ~ 64 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Frizzled-9 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 571-620 of Human Frizzled-9.

Conjugate: Unconjugated

Modification: Unmodified