Product Description

FTO Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90539-50, BS90539-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9C0B1(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC

All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200

Background: FTO, also known as Fatso or KIAA1752, is a 505 amino acid protein that has an N-terminal nuclear localization signal. Expressed in a variety of tissues, with highest levels present in brain and pancreatic tissue, Fatso exists as four alternatively spliced isoforms, one of which is associated with a predisposition to childhood and adult obesity. Due to its involvement in the development of obesity, Fatso is associated with an increased BMI and may be involved in the pathogenesis of type 2 diabetes. The gene encoding Fatso maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: FTO polyclonal antibody detects endogenous levels of FTO protein.

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of FTO on different lysates using anti-FTO antibody at 1/1,000 dilution. Positive control: Lane 1: Human fetal brain tissue
Lane 2: HepG2
Lane 3: 293, Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FTO antibody. Counter stained with hematoxylin.