FUBP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90540-50, BS90540-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q96AE4(Human) Q91WJ8(Mouse) Q32PX7(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Activation of FUSE, the far upstream element, is required for the proper ex-pression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP1 (FUSE-binding protein or far upstream element-binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL-60 cells, FBP1 (FBP), FBP2 and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP1 is detected in undifferentiated cells and is substantially decreased following cellular differentiation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: FUBP1 polyclonal antibody detects endogenous levels of FUBP1 protein.
Bioworld Molecular Weight: 74 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Raji, ICC staining FUBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: FBP, FUSE-binding protein 1, hDH V, FUBP1, DNA helicase V, far upstream element (FUSE) binding protein 1, Far upstream element (FUSE) binding protein 4, Far upstream element binding protein 1, far upstream element binding protein, Far upstream element-binding protein 1, FBP, FUBP, Fubp1, FUBP1, Fubp4, FUSE binding protein 1, FUSE-binding protein 1, HDH V,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified