FUS/TLS Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90542-50, BS90542-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35637(Human) P56959(Mouse) Unigene:100218(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: EWS and FUS/TLS are nuclear RNA-binding proteins. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1, in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various Ets transcription factors, including Fli-1, Et V1 and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. In human myxoid liposarcomas and myeloid leukemias, chromosomal translocation results in the fusion of the N-terminal region of FUS/TLS with the open reading frame of CHOP. In normal cells, FUS/TLS binds to the DNA-binding domains of nuclear steroid receptors and is also present in subpopulations of TFIID complexes, indicating a potential role for FUS/TLS in the processing of primary transcripts that are generated in response to hormone-induced transcription.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: FUS/TLS polyclonal antibody detects endogenous levels of FUS/TLS protein.
Bioworld Molecular Weight: 75 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of FUS/TLS on K562 cells lysates using anti-FUS/TLS antibody at 1/1,000 dilution., ICC staining FUS/TLS in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: FUS, 75 kDa DNA pairing protein, 75 kDa DNA-pairing protein, ALS6, Amyotrophic lateral sclerosis 6, fus, FUS CHOP, Fus like protein, FUS, FUS1, Fused in sarcoma, Fusion (involved in t(12; 16) in malignant liposarcoma), Fusion derived from t(12; 16) malignant liposarcoma, Fusion gene in myxoid liposarcoma, Heterogeneous nuclear ribonucleoprotein P2, hnRNP P2, hnRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, RNA-binding protein FUS, TLS, TLS CHOP, Translocated in liposarcoma, Translocated in liposarcoma protein,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified