FXR2 (E576) Polyclonal Antibody-BS2008

FXR2 (E576) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2008-50, BS2008-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51116

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC/IF: 1:50~1:200
IP: 1:10~1:100

Background: The FMR1 protein (or FMRP) is an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. It contains several features that are characteristics of RNA-binding proteins, including two hnRNPK homology (KH) domains and an RGG amino acid motif (RGG box). FMR1 can also interact with two fragile X syndrome related factors, FXR1 and FXR2, and these proteins form heterodimers through their N-terminal coilcoiled domains. FMR1 localizes to both the nucleus and the cytoplasm, and since it contains both a nuclear localization signal and a nuclear export signal it is also implicated in the nucleo-cytoplasmic transport of mRNAs.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FXR2 (E576) polyclonal antibody detects endogenous levels of FXR2 protein.

Bioworld Molecular Weight: ~ 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FXR2 (E576) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: H9C2 whole cell lysate (40ug)
Lane 4: MEF whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of FXR2 (E576) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunoprecipitation of HEK293T cell lysate using FXR2 (E576) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3). Lane 1 is 30% input. The western blot was probed using FXR2 (E576) #BS2008. “↑”（supernatant); “↓(deposition), Immunohistochemistry (IHC) analyzes of FXR2 (E576) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Fragile X mental retardation syndrome-related protein 2; FMR1L2; FXR2

Immunogen: Synthetic peptide, corresponding to amino acids 550-600 of Human FXR2.

Conjugate: Unconjugated

Modification: Unmodified