Fyn Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90546-50, BS90546-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P06241(Human) P39688(Mouse) Q62844(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Src is the human homolog of the v-src gene of the Rous sarcoma virus, also called avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking. Fyn and Lck kinases play a key role in T-cell antigen receptor (TCR) signaling. The human Fyn gene maps to chromosome 6q21 and encodes a 537 amino acid protein.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Fyn polyclonal antibody detects endogenous levels of Fyn protein.
Bioworld Molecular Weight: 60 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Fyn on HUVEC cell using anti-Fyn antibody at 1/200 dilution., ICC staining Fyn in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: SLK, FYN, C syn protooncogene, Fyn, FYN oncogene related to SRC FGR YES, FYN, OKT3 induced calcium influx regulator, P59 FYN, p59-Fyn, Protein tyrosine kinase fyn, Proto oncogene tyrosine protein kinase fyn, Proto-oncogene c-Fyn, Proto-oncogene Syn, Protooncogene Syn, SLK, Src like kinase, Src yes related novel gene, Src-like kinase, Src/yes related novel, SYN, Tyrosine kinase p59fyn T, Tyrosine kinase p59fyn(T), Tyrosine-protein kinase Fyn,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified