Gab1 (E655) Polyclonal Antibody-BS1539

Gab1 (E655) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1539-50, BS1539-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13480

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Gab 1 (GRB2-associated binder 1), one such member of the IRS family, plays an important role in cellular growth response, transformation and apoptosis. Gab 1 is a multi-substrate docking protein that functions downstream in the signaling pathways of different receptor kinases, including EGFR. Gab1 is tyrosine phosphorylated normally in response to insulin and consequently enhances phosphatidylinositol 3- kinase (PI3K) binding. In response to osmotic shock, tyrosine-phosphorylated Gab 1 (p-Gab 1) also binds and activates phosphatidylinositol 3-kinase, suggesting that Gab 1 is the major site for PI3K recruitment following osmotic shock stimulation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Gab1 (E655) polyclonal antibody detects endogenous levels of Gab1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Gab1 (E655) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of Gab1 (E655) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 76, 110 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1; GAB1

Immunogen: Synthetic peptide, corresponding to amino acids 622-672 of Human Gab1.

Conjugate: Unconjugated

Modification: Unmodified