Product Description

GAB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90549-50, BS90549-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13480(Human)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc and Gab 1 also participate in insulin signaling by linking the insulin receptor to Ras by forming complexes with GRB2 (another adapter protein) and Sos independently of IRS-1. Gab 1 is also thought to be involved in the EGF receptor signaling pathway.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GAB1 polyclonal antibody detects endogenous levels of GAB1 protein.

Bioworld Molecular Weight: 77 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution., ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GAB1, Gab 1, GAB1, GAB1, GRB 2 associated binder 1, GRB 2 associated binding protein 1, GRB2 associated binding protein 1 isoform a, GRB2 associated binding protein 1 isoform b, GRB2-associated binder 1, GRB2-associated-binding protein 1, Growth factor receptor bound protein 2-associated protein 1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified