Product Description

Gab2(Ab-623)Polyclonal Antibody

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65238-50, BS65238-100

Swiss-Prot: Q9UQC2

Host: Rabbit

Gab2(Ab-623)Polyclonal Antibody-BS65238

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Background: GRB2 associated binding protein 2(GAB2) Homo sapiens This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity: Phospho-Gab 2 (Y643) Polyclonal Antibody detects endogenous levels of Gab 2 protein only when phosphorylated at Y643.

Bioworld Molecular Weight: ~ 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry analysis of paraffin-embedded human brain, using Gab2 (Phospho-Tyr643) Antibody. The picture on the right is blocked with the phospho peptide., Western blot analysis ofLysates from Jurkat cells treated with IFN 2500U/ML 30', using Gab2 (Phospho-Tyr643) Antibody. The lane on the right is blocked with the phospho peptide.

Alternative Name: GAB2; KIAA0571; GRB2-associated-binding protein 2; GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; pp100

Immunogen: The antiserum was produced against synthesized peptide derived from human Gab2 around the phosphorylation site of Tyr643. AA range:609-658

Modification: Unmodified

Conjugate: Unconjugated