Product Description

GAD-65/67 (D572) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1400-50, BS1400-100

Sizes: 50µl, 100µl

Swiss-Prot: Q99259/Q05329

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GAD-65/67 (D572) polyclonal antibody detects endogenous levels of GAD65 and GAD67 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GAD-65/67 (D572) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of GAD-65/67 (D572) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 65, 67 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD 65; Glutamate decarboxylase 65 kDa isoform; GAD2; GAD-65; GAD 2; GAD-2; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD 67; Glutamate decarboxylase 67 kDa isoform; GAD1; GAD; GAD67; GAD-1; GAD 1

Immunogen: Synthetic peptide, corresponding to amino acids 540-590 of Human GAD-65.

Conjugate: Unconjugated

Modification: Unmodified