Product Description

Gads (N158) Polyclonal Antibody

Catalogue Numbers: BS3870-50, BS3870-100

Sizes: 50µl, 100µl

Alternative Name: GRB2-related adapter protein 2; Adapter protein GRID; GRB-2-like protein; GRB2L; GRBLG; GRBX; Grf40 adapter protein; Grf-40; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein; GrpL; Protein GADS; SH3-SH2-SH3 adapter Mona; GRAP2; GADS; GRB2L; GRID

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75791

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Src homology 3 (SH3) region is a small protein domain of approximately 60 amino acids present in a large group of proteins. In general, it exists in association with catalytic domains, as in the nonreceptor protein-tyrosine kinases and phospholipase C-γ, within structural proteins, such as spectrin or myosin, and in small adapter proteins, such as Crk and GRB2. SH3 domains are often accompanied by SH2 domains of 100 amino acids that bind to tyrosine-phosphorylated regions of target proteins, frequently linking activated growth factors to putative signal transduction proteins. Deletion or mutation of SH3 domains generally activates the transforming potential of nonreceptor tyrosine kinases, suggesting that SH3 mediates negative regulation of an intrinsic transforming activity. Gads is an adapter proteins that contains both SH2 and SH3 domains. Gads binds to tyrosine-phosphorylated proteins, such as Shc, and functions to couple these proteins to downstream effectors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Gads (N158) polyclonal antibody detects endogenous levels of Gads protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Gads (N158) pAb at 1:1000 dilution
Lane 1: CT26 whole cell lysate(40ug)
Lane 2: PC12 whole cell lysate(40ug)
Lane 3: SGC7901 whole cell lysate(40ug)
Lane 4: H1792 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 131-180 of Human Gads.

Conjugate: Unconjugated

Modification: Unmodified