Product Description

Galectin-3 (N174) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1137-50, BS1137-100

Sizes: 50µl, 100µl

Swiss-Prot: P17931

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. galectin-3 mediates the endocytosis of β-1 Integrins in a lactose-dependant manner and is associated with thyroid malignancy and Crohn’s disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Galectin-3 (N174) polyclonal antibody detects endogenous levels of Galectin-3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Galectin-3 (N174) polyclonal antibody at 1:500 dilution
Lane 1: THP-1 whole cell lysate (40ug)
Lane 2: L02 whole cell lysate (40ug)
Lane 3: HCT116 whole cell lysate (40ug)
Lane 4: HepG2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Galectin-3 (N174) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CBP35; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3; MAC2; Galectin 3; Galectin3

Immunogen: Synthetic peptide, corresponding to amino acids 140-190 of Human Galectin-3.

Conjugate: Unconjugated

Modification: Unmodified