Galectin 3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90555-50, BS90555-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P17931 Human;P16110 Mouse;P08699 Rat
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) inter- actions and play a role in tumor progression, pre-mRNA splicing and apop-tosis. The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. Galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. Galectin-3 mediates the endocytosis of β1 Integrins in a lactose-dependent manner and is associated with thyroid malignancy and Crohn's disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: Galectin 3 polyclonal antibody detects endogenous levels of Galectin 3 protein.
Bioworld Molecular Weight: 26 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Galectin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Mouse colon tissue lysate, ICC staining Galectin 3 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ER1803-82 at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Alternative Name: Gal-3, CBP 35, GALBP, LGALS3, 35 kDa lectin, Carbohydrate binding protein 35, Carbohydrate-binding protein 35, CBP 35, CBP35, Gal-3, GAL3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin 3 internal gene, included, Galectin-3, Galectin3, GALIG, GBP, IgE binding protein, IgE-binding protein, L 31, L 34, L-31, L-34 galactoside-binding lectin, L31, Laminin-binding protein, Lectin L-29, Lectin, galactose binding, soluble 3, LEG3, LGALS2, LGALS3, MAC 2 antigen, Mac-2, Mac-2 antigen, MAC2, Macrophage galactose-specific lectin, MGC105387 antibody
Immunogen: Recombinant protein within Human Galectin 3 aa 1-180.
Conjugate: Unconjugated
Modification: Unmodified